Authors: Daniela-Rodica Mitrea, Malkey Ronny, Nadina-Liana Pop, Adriana Filip, Simona Clichici, Remus Moldovan, Cristina Bidian, Alina-Mihaela Toader, Teodora-Larisa Florian
“Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Department of Physiology
Affiliation
“Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Department of Physiology
Abstract
Background. Carrageenan-induced inflammation implies the release of inflammatory cytokines and also, oxidative stress, with high amounts of reactive oxygen species (ROS) production.
Aims. The present study aimed to investigate the effects of a unique dose of chlorogenic acid, orally administered at one hour after a paw inflammation was produced.
Methods. The paw edema and oxidative stress parameters in rats that were injected with 0.2 ml carrageenan solution 1% for paw inflammation production were investigated, and after 1 hour they received a single dose of either chlorogenic acid (100 mg/kg or 150 mg/kg) or indomethacin (1 mg/kg), by oral gavage. The paw edema was investigated using the
plethysmometer test (at 2, 6 and 24 hours after carrageenan injection) and oxidative stress by spectrophotometry.
Malondialdehyde (MDA), glutathione (GSH) and oxidized glutathione (GSSG) levels were investigated from blood samples, paw inflamed skin and kidneys.
Results. The present study showed that chlorogenic acid (both doses) and indomethacin produced significant decreases of inflamed paw volume, compared to the control group (rats without treatment). Lipid peroxidation was reduced significantly and the antioxidant protection investigated through the GSH/GSSG ratio was increased significantly in all rats that received medication, in all the tested samples: inflamed skin, serum, kidneys.
Conclusions. Chlorogenic acid may reduce efficiently oxidative stress and edema if it is administered during the first phase of carrageenan-induced inflammation.
Keywords: chlorogenic acid, oxidative stress, inflammation, antioxidants, carrageenan, green coffee
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